Lucie Himmlova
Charles University, Czech Republic
Title: The response of HMSC-bm, SaOS-2 and imunocompetent cells to calcium phosphate doped alginate coatings on titanium surfaces
Biography
Biography: Lucie Himmlova
Abstract
The release of calcium and phosphate ions into the interface around orthopedic and dental implants may improve the bone healing. Human mesenchymal stem cells derived from the bone marrow (HMSC-bm), imunocompetent (mononuclear) cells obtained from buffy coat and connective tissue cell lines SaOS-2 were used for testing of alginate hydrogel layers doped with hydroxyapatite (Ti/ALG/HAP) or beta-tricalcium phosphate (Ti/ALG/TCP) immobilized onto titanium (Ti) surfaces. The pristine Ti and alginate (ALG) served as control surfaces. Cytokine production was assessed by multiplex proteomic analysis RayBio Human Inflammation Array (RayBiotech, USA) after 3 and 7 days cultivation. Each cell type produced a different spectrum of cytokines. Mononuclear cells produced preferentially factors of nonspecific immunity (MIP, RANTES, MCP-1, IL-6 and IL-8) in doses higher than detected for the positive control of the RayBio array. The cytokine production declined in the order Ti/ALG/HAP --->Ti --->Ti/ALG/TCP ---> Ti/ALG. HMSC-bm produced mostly chemokines activating predominantly chemotaxis and activating monocytes, granulocytes and neutrophils, but in doses lower than mononuclear cells. SaOS-2 cells produced the broadest spectrum of cytokines but in low doses and with no significant difference between Ti/ALG/HAP and Ti/ALG/TCP surfaces. The immune response of mononuclear and stem cells showed differences between materials, whereas SaOS-2 cells weren’t sufficiently sensitive. Therefore, besides SaOS-2 cells, HMSC-bm and mononuclear cells should be also considered for in vitro evaluation of overall inflammatory response induced by presence of the implant.
