Ming Yue Lee
Arizona State University, USA
Title: Serial crystallography of a G-protein coupled receptor using polychromatic synchrotron radiation source
Biography
Biography: Ming Yue Lee
Abstract
Since the first successful serial crystallography (SX) experiment at synchrotron radiation sources, the popularity of this approach has continued to grow, showing that 3rd generation synchrotrons can be viable alternatives to scarce X-ray free electron laser sources. Synchrotron radiation flux may be increased about 100 times by a moderate increase in bandwidth (“pink beam” conditions) at some cost in data analysis complexity. Here, we report the first high-viscosity injector-based pink beam SX experiments. The structures of A2A adenosine receptor (A2AAR) and proteinase K (PK) were determined to 4.2Å and 1.8Å resolution using 24 and 4 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR required an alternative data processing strategy. This demonstration of the feasibility presents new opportunities for the time-resolved experiments with micro-crystals to study structural changes in real-time at pink beam synchrotron beamlines worldwide